Aberrant pistils leads to reduced fertility in strong BnAP2-RNAi plants.

Reciprocal crosses between wild-type plants and strong ap2 mutant plants were performed on secondary inflorescences. Plants were grown together in the same conditions. Three flowers at identical positions (11th to 13th flowers for wild type or male-sterile mutant, 10th to 15th flowers in ap2 mutants) were manually pollinated. Two to four inflorescences per plant and four to five plants were used for calculations. Similar results were obtained in an independent experiment that was performed in a different season of the year. Means ± SD are shown.