Comparison of Leaves and Stem Aqueous Extract of Tridax Procumbens for Antimicrobial Activity

In present study, aq. extracts of leaves and stem part of Tridax Procumbens were compared for antimicrobial activity. Firstly, all organoleptic and physicochemical properties of leaves and stem powder were evaluated and it shows that drug is pure and having required constituents sufficiently. Aq. extract of leaves powder and stem powder was prepared separately using Soxhlet extraction technique. Both the extracts were evaluated for physiochemical screening using standard procedures. Aq. extract of leaves shows presence of tannis, saponins, anthocyanin, coumarins, alkaloids, proteins, amino acids, diterpenes, phytosterol, cardial glycosides, phlobatannins and flavonoids. Aq. extract of stem shows presence of tannins, coumarins, phenol, cardial glycosides and flavonoids. Both extracts were primarily evaluated for antimicrobial activity against E.coli and S. aureus by selecting dose of 500 mg. Finally, antimicrobial assay was performed for both extracts (500mg) against E.coli and S. aureus by well diffusion method using Amoxicillin and Amikacin as standards respectively. Antimicrobial assay shows that aq. extract of stem part is more effective against S. aureus than E.coli; leaves aq. extract also shows antimicrobial activity against S .aureus and E.coli. From present studies we can conclude that aq. extract of stem and leaves of Tridax Procumbens having antimicrobial activity and stem extract is more effective against S. aureus as compared to leaf extract.