Agropyron_cristatum_scaffolds

Agropyron cristatum is a wild crop relative what makes this species
attractive as a possible donor of genes for crop improvement. This species
is well known for its resistance and tolerance genes. We sequenced
individual chromosomes of Agropyron cristatum cv. Parkway and its
wheat-Agropyron addition lines to find simple sequence repeat markers that
could help breeders in their breeding programs.  Firstly, we used
flow cytometry sorting of studied plant material, which is the
well-established approach in our laboratory. We sorted individual
chromosomes (1P - 7P) of Agropyron followed by amplification of their DNA
using two different amplification methods. Libraries for sequencing were
prepared and sequencing was done on HiSeq and MiSeq Illumina
instruments.  Secondly, we used the datasets for de novo
assembly, SSR markers development and their verification via PCR method
with appropriate primers. Other usage of the sequences was to study
orthologous relationship between Agropyron and wheat subgenomes and we
also looked at representation of genic and non-genic sequences in
Agropyron itself. Stored datasets are assembled scaffolds of each
Agropyron chromosomes, that could be of a high value in breeding studies
for the characterization of a progeny or a breeding material
itself.