Additional file 1 of Mechanisms of metabolic adaptation in the duckweed Lemna gibba: an integrated metabolic, transcriptomic and flux analysis

Additional file 1: Table S1. Increase in fronds area (cm2) during culture time (hours). Means and standard deviations represented three independent replicates. Table S2. Metabolite analysis by Liquid Chromatography / Mass Spectrometry. See also Table S13. Table S3. Biomass composition data (weight % in dry weight).  Means and standard deviations represented three independent replicates. Table S4. Measurements of 13C-enrichment in metabolites by GC/MS. Table S5. Measurement of 13C-enrichment in total frond biomass by Isotope-Ratio Mass Spectrometry. L. gibba fronds were grown with medium glucose consisting of 40% U13C6 glucose (99% 13C) and 60% unlabeled glucose (1.1% natural 13C abundance). Table S6. Definition of carbon flux model with indication of change in gene expression and change in flux. Table S7. Metabolite names used in the flux model. Table S8. Assessment of step wise model improvement. The four model configurations (A, B, C) correspond panels A, B and C in Fig. S1, respectively. Table S9. Best fit flux results for inorganic nitrogen condition (INS) and organic nitrogen condition (ONS). Table S10. Flux values with statistical confidence measures. Table S11. Differential expression analysis data (Outputs of DESeq package, A= INS condition, B= ONS condition). Table S12. List of Uniprot accession numbers associated with Lemna gibba protein IDs (information retrieved on April 4, 2018, http://www.uniprot.org). Table S13. GO term enrichment analysis for significantly differently expressed genes of Lemna gibba. Table S14. Mass spectrometer parameter settings for analysis of medium substrate levels. Table S15. Mass spectrometer parameter settings for metabolites measurements. Liquid chromatography coupled to mass spectrometer. Table S16. Primer sequences for qRT-PCR.