BEHAV3D: A 3D live imaging platform for comprehensive analysis of engineered T cell behavior and tumor response

The use of patient-derived material and immune cell co-cultures in
modeling immune-oncology has gained significant interest for understanding
and manipulating immune cell tumor targeting in a patient-specific
context. However, current protocols have limitations in visualizing and
analyzing the dynamic cellular features of these living culture systems.
We recently developped a workflow names BEHAV3D,  that combines
multi-color live 3D imaging and computational tools to analyze cell death
dynamics, classify T cell behavior, and generate data-informed 3D images
and videos. Here we provide some example pre-processed dataset of videos
of two co-culture set ups: breast cancer Patient Derived Organoids with αβ
T cells engineered to express a γδ TCR (TEGs) and Acute Lymphoblastic
Leukemia cells with CD19 CART cells.