Mus musculus Raw sequence reads

A total of 6 brain samples were sequenced on DNBSEQ platform to perform small RNA sequencing and compare the difference in gene expression. The samples were collected from littermates of a specific gene-knockout mice, in which ZSWIM8 was specifically deleted in central nervous sysytem by Nestin-cre. Whole brains were dissected from Zf/f and Zf/f;Nestin-Cre littermates at P2 and frozen in liquid nitrogen immediately. Sample processing and sRNA-Seq was done by BGI Genomics (Shenzhen, China). DNBSEQ was used for sequencing and clean data were aligned to the mouse reference genome (GRCm38/mm10) using HISAT and to sRNAs in miRbase (https://www.mirbase.org). miRDeep2 was used to predicted newly discovered miRNAs. miRNA expression was quantified with Unique Molecular Identifiers (UMI), and DEGseq was used for analysis of differentially expressed genes defined as | log2(fold-change) | > 0 and Padj < 0.05 (Benjamini-Hochberg).