Ip, wallace (2010) CIL:6264, Oryctolagus cuniculus, skeletal muscle cell. CIL. Dataset

Rabbit psoas skeletal muscle fibers were blended in the presence of 5 mM MgATP to dissociate thick and thin filaments. The medium was then gently replaced with 0.3M KCl to dissociate thick filaments. Dissociated filaments were adsorbed onto mica flakes (Heuser JE, J Mol Biol. 1983 Sep 5;169(1):155-95), quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 11 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

This image was recorded on Kodak 4489 film at 75,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:6263.

本研究采用5 mM MgATP将兔子的腰大肌骨骼肌纤维进行混合，以分离粗肌丝和细肌丝。随后，用0.3M KCl轻柔地替换介质，以进一步分离粗肌丝。分离后的肌丝被吸附于云母片上（Heuser JE，J Mol Biol. 1983年9月5日；169(1):155-95），并在Heuser型冷冻压裂机中与液氦冷却的铜块接触快速冷冻，之后在Balzers 400冷冻断裂机上进行了冷冻蚀刻。样品随后以11度角进行铂-碳复制，并在100 keV的JEOL 100CX2透射电子显微镜下进行观察。该图像记录于柯达4489胶片上，放大倍数为75,000倍。在+5度和-5度角处拍摄了立体对图像。胶片使用尼康Coolscan 9000ED扫描仪以20微米像素间距进行扫描。与CIL:6263对应的立体对图像。