CRISPR-PAINT nanorulers
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The GATTA-PAINT 40 RG nanoruler (GATTAquant, DNA nanotechnologies) consists of three fluorophores (ATTO 655/ATTO 542) separated 40 nm from each other. Imaging of the sample was performed on an Olympus IX-81 inverted microscope equipped with an Olympus UApo N 100X/1.49 NA, oil-immersion objective in TIRF illumination mode (Olympus, cellTIRF Illuminator) with ~200 nm evanescence field depth. Sample excitation was provided by 488 nm and 561 nm lasers at 23.1 mW laser power. 300 total images were collected with an EM-CCD sensor (Andor iXon 897), exposure time set to 50 ms per frame and a pixel size of 160 nm.
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Zenodo创建时间：
2022-08-03
