S2 Data -

Expression and statistical results from transcriptomic analysis presented in Fig 4. Sheet 1. Haemoglobin_getmm. GeTMM-normalised expression and gene IDs for Hb (alpha and beta subunits and paralogs i, ii, and iv), Mb, Cytgb, and Ngb for each individual larval anemonefish at ages 4, 6, and 9 dph. These data are the same as S1 Data sheet “Fig 3” but include Ensembl gene IDs. Sheet 2. Raw_counts. Raw, nonnormalised gene counts for all genes from the FeatureCount pipeline (Fig 4A). Sheet 3. all_degs_getmm. GeTMM-normalised expression and gene IDs (with abbreviations and descriptions) for genes identified as DEGs, for each individual fish larvae across 3 age groups (4, 6, and 9 dph) (Fig 4B). Sheet 4. Heatmap_toptags. GeTMM-normalised expression and gene IDs (with abbreviations and descriptions) for the top most significant genes across 3 age groups (4, 6, and 9 dph), used to create Heatmap (Fig 4D). Sheet 5. Volcanoplot_toptag. Mean across all groups (“baseMean”), log10 of the mean (“log10basemean_amel”), log of the fold-change (“log2FoldChange”), error estimate of the log fold-change (lfcSE), significance (“pvalue”), and adjusted p-value (“padj”) for the top most significant DEGs when comparing 9 to 4 dph, used to create Volcano plot (Fig 4E). Sheet 6. GOs. GO enrichment analyses (of the significantly up- or down-regulated genes when comparing 9 dph and 4 dph. Data include the fold enrichment (how many more times than expected did a gene belonging to a given GO category appear in the list of DEGs) and the adjusted p-value (FDR) (Fig 4F). Sheet 7a. 6dph_vs_4dph, 7b. 9dph_vs_4dph, 7c. 9dph_vs_6dph. Mean across all groups (“baseMean”), log10 of the mean (“log10basemean_amel”), log of the fold-change (“log2FoldChange”), error estimate of the log fold-change (lfcSE), significance (“pvalue”), and adjusted p-value (“padj”) for all DEGs identified when comparing 9 dph and 4 dph, 9 dph and 6 dph, and 6 dph and 4 dph. Sheet 8a. down_unique_6dph_vs_4dph, Sheet 8b. up_unique_6dph_vs_4dph. Mean across all groups (“baseMean”), log10 of the mean (“log10basemean_amel”), log of the fold-change (“log2FoldChange”), error estimate of the log fold-change (lfcSE), significance (“pvalue”), and adjusted p-value (“padj”) for DEGs that were unique when comparing 6 dph and 4 dph, i.e., down or up at 6 dph but not 9 dph. Sheet 9a. down_unique_9dph_vs_6dph, Sheet 9b. up_unique_9dph_vs_6dph. Mean across all groups (“baseMean”), log10 of the mean (“log10basemean_amel”), log of the fold-change (“log2FoldChange”), error estimate of the log fold-change (lfcSE), significance (“pvalue”), and adjusted p-value (“padj”) for DEGs that were unique when comparing 9 dph and 6 dph, i.e., down or up at 9 dph but not at 6 dph.
(XLSX)