Genome assembly of Nicotiana tabacum L. ZY300

The final assembly included 4.17 Gb of sequences with a contig N50 of 27.17 Mb, and 96.98% of the sequences were anchored to 24 pseudochromosomes. The completeness of the assembled genomes was supported by a high mapping rate (99.72%), high genome coverage of Illumina short reads (99.84%) and single-copy orthologues from the Benchmarking Universal Single-Copy Orthologues (BUSCO) analysis (99.3%). The high base accuracy of the assembled genomes was indicated by base pair quality values of 57.09 and k-mer completeness values of 99.25%. Compared with previous genomes assembled using short reads, our assembly showed substantial improvements in assembly size, continuity and correctness