Statistical significance test results for interaction effects (binning and simulation parameter) on ASTRAL.

We performed ANOVA to test the significance of whether there is an interaction between the choice of the method (unbinned, weighted binned, unweighted binned, WSB-50: weighted statistical binning using 50% bootstrap support threshold and WSB-75: weighted statistical binning using 75% bootstrap support threshold) and the variable changed in each dataset. For weighted vs. unweighted, we compared 50% bootstrap support threshold for avian, 75% for mammalian, and both 50% and 75% for 15- and 10-taxon datasets. All p-values are corrected for multiple hypothesis testing using the FDR correction (n = 17). “n.a.” stands for “not available”.