Small RNAs in Arabidopsis thaliana and its RISC complexes

RNA populations in Arabidopsis thaliana and its RISC complexes Keywords: Highly parallel pyrosequencing Small RNAs were prepared from Arabidopsis total extracts, AGO1 and AGO4 complexes, ligated to a 3' adaptor and a 5' acceptor sequentially, and then RT-PCR amplified. PCR products were reamplified using a pair of 454 cloning primers and provided to 454 Life Sciences (Branford, CT) for sequencing. For technical details, see Qi, Y., He, X., Wang, X.J., Kohany, O., Jurka, J., and Hannon, G.J. 2006. Distinct catalytic and non-catalytic roles of ARGONAUTE4 in RNA-directed DNA methylation. Nature 443(7114): 1008-1012.