Assembly of combinatorial antibody libraries on phage surfaces: the gene III site.
收藏PubMed Central1991-09-15 更新2026-05-16 收录
下载链接：
https://pmc.ncbi.nlm.nih.gov/articles/PMC52428/
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资源简介：
A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carboxyl-terminal domain of the gene III protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 10(3)- to 10(5)-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1991-09-15
