Datasets of endodontic microorganisms killed at 265 and 280 nm wavelengths by ultraviolet C light emitting diodes

Treatment of root canals with ultraviolet C (UVC) light-emitting diodes (LED) is a promising adjunct to current root canal disinfection procedures that are essential for preserving the health and the healing of periapical tissues. Here, we hypothesized that UVC LED treatment would kill microorganisms associated with endodontic infections and kill microorganisms in instrumented root canal models.

Three different UVC LED units were constructed. One unit emitted 265 nm from a 12 mW LED, one unit emitted 265 nm from a 22.5 mW LED, and one unit emitted 280 nm from an 8 mW LED. Power levels emitted from the three units were measured without and with fiberoptic (FO) filaments using an energy meter (PM100D, Thorlabs, Inc., Newton, NJ USA) with a standard photodiode sensor (S120VC, 200-1100 nm, 50 mW; Thorlabs, Newton, NJ USA) for 30 seconds.

The raw data from the energy meter readings for these 3 units is listed in Table 1. This includes the wavelength the meter used to record the data for each unit (Wavelength read at (nm)), the diameters of the hub opening or FO filament emitting the UV light (Diameter of hub opening or FO filament (mm)), the minimum power value recorded from the meter (Minimum value (µW)), the maximum power value recorded from the meter (Maximum value (µW)), the mean value recorded from the meter (Mean value (µW)), the standard deviation of the mean value recorded from the meter (Standard deviation (µW)), the number of meter readings (Number of readings (n)), and the time of recording (Time (sec)).

The raw data from the antimicrobial activities of these 3 units on Candida albicans ATCC 64124, Enterococcus faecalis ATCC 29212, Streptococcus sanguinis ATCC 10556, Staphylococcus aureus ATCC 29213, and methicillin-resistant S. aureus (MRSA #7) treated in vitro on discs in laboratory assays is listed in Table 2. This includes the length of time each microorganism was treated (Group (seconds)), the UVC LED unit used to treat each microorganism (UVC LED treatment), the replication number (Replication), the microorganism treated (Microorganism), and the number of viable colony forming units (CFU) of microorganisms surviving UVC LED treatment (Viable CFU).

The raw data from the antimicrobial activity of the 265 nm (22.5 mW) unit on E. faecalis ATCC 29212, C. albicans ATCC 64124, MRSA #7, or S. aureus ATCC 29213 in ex vivo models of root canals is listed in Table 3. This includes whether the group was a control or treatment (Group), if there was UVC LED treatment with the 265 nm (22.5 mW) unit (UVC LED treatment), the replication number (Replication), the microorganism treated (Microorganism), and the number of viable CFU of microorganisms surviving 265 nm (22.5 mW) unit treatment (Viable CFU).