Genome binding of SMARCA2 and SMARCA4 in lung cancer cells reconstituted with SMARCA4 WT with and without SMARCA2 depletion

Using ChIP-seq, we identified the genome-wide occupancy of SMARCA2 and SMARCA4 in lung cancer cells (NCI-H1944) reconstituted with SMARCA4 WT. We also determined how SMARCA4 occupancy changed in NCI-H1944 after SMARCA2 depletion. NCI-H1944 cells stably expressing a doxycycline-inducible SMARCA2-targeting shRNA were lentivirally transduced with LACZ and SMARCA4 WT and selected with blasticidin for 5 d. Cells were treated with vehicle or 0.5 ug/mL doxycycline for 4 d to obtain significant SMARCA2 knockdown. Cells were fixed, quenched and washed for ChIP.