Transcriptomic profiling of cultured CD8+ T cells from mice and treated with either IL2, IL2/SIINFEKL(TCR), IL2/IL4, or IL2/IL4/SIINFEKL(TCR)

The plasticity of CD8 cells lend itself well to understanding underlying molecular mechanism for cell identity and downstream effects of microenvironment exposures. CD8 T cells in the context of memory has been studied at the epigenomic level; however, responses after immunologic microenvironment exposures culminating in a final receptor stimulation readout has not been studies in detail. We use RNA-seq to explore these questions. RNA-sequencing of TCR activated (SIINFEKL) and unactivated CD8+ T cells purified from OT1 Mouse spleens and cultured in the presence of IL-2 or IL-2/IL-4 for 4 Days.