Genome-wide maps of chromatin openness in control and LMNA R527C mutant MSCs

We performed ATAC-seq to examine to chromatin of openness of coding genes in healthy control and LMNA R527C patient-derived MSCs. The reduction of LADs did not increase the accessibility of the promoters flanking coding genes. The genome wide analysis of ATAC-seq showed that openness of coding genes related chromatin was decreased in mutant MSCs.Thus, R527C mutant Lamin A led to dysfunction of LADs and dysregulation of gene transcription, probably, leading to slow down or arrest of cell proliferation and differentiation. LADs were distributed across heterochromatic, promoter, and actively transcribed regions of the genome and were associated with DNA methylation and suppressed gene expression. We thus performed ATAC-seq to examine to chromatin of openness of coding genes in control and LMNA R527C mutant MSCs as Lamin A-LADS binding affinity were changed during LMNA R527C mutation. Examination of chromatin openness in control and LMNA R527C mutant MSCs.