Quantitative analysis of LIP retinal damage.

Total numbers of cones automatically quantified in retinal wholemounts from experimental rats whose left eye had been exposed to Light Emitting Diode-induced phototoxicity (LIP) and were analysed seven days later. The numbers of cones within predetermined fixed-size circular areas (PCA) were also automatically quantified. The total areas of the retinas (Area of retina) as well as the areas lacking L- or S-cones (Area of damaged region) were measured over flat-mounted retinas. The maximum diameters (Diameter length of damage) of the areas lacking L- or S-cones were measured over flat-mounted retinas and also the length of ONL lacking cell nuclei was measured in vivo with OCT. Statistical analysis: *T-test p = 0.369; †T-test p = 0.049; ‡T-test p≤0.001; §T-test p≤0.001; # When compared with the areas of naïve retinas (see Table 2) there were no significant differences (ANOVA p = 0.974).Quantitative analysis of LIP retinal damage.