Changes in global open chromatin structure caused by acute deletion of TERT in spermatogonial stem cells. Changes in global open chromatin structure caused by acute deletion of TERT in spermatogonial stem cells

To understand the catalytic activity-independent functions of TERT in global chromatin structure, we acutely deleted TERT in spermatogonial stem cell (SSC) populations in the testis, and performed ATAC-seq. We find significant global loss of open chromatin structure in TERT-deleted SSC populations. Overall design: TERT was acutely deleted by tamoxifen-dependent Cre-LoxP system, and ATAC-seq libraries were prepared from testicular cells purified by FACS.