Transcriptome-wide identification of STING agonist programed B cells

We employed RNA-seq to study the transcriptomic change in B lymphocytes in response to STING agonist. Transcriptomic analysis of wild type and TMEM173–/– B cells stimulated with STING agonist revealed that the inflammatory response pathway enriched in 3'3'-cGAMP treated WT B cells but lacking in Tmem173-/-B cells comprised of a cluster containing inhibitory regulators, provoking STING signal to evade immune surveillance by inducing an immunosuppressive B cell population. Overall design: The primary B cells were isolated from WT, Tmem173-/- mice, and then treated with 3'3'-cGAMP for 12 hours. The total RNA was extracted and applied to RNA-Seq analysis.