miRNA signatures derived from plasma small extracellular vesicle as predictors for organo-tropic metastasis of gastric cancer

Metastasis is a major problem of gastric cancer. In this study, small extracellular vesicle (sEV)-derived miRNAs were sequenced to screen biomarkers for GC’s organo-tropic metastasis. Plasma from 40 treatment-naïve gastric cancer patients including 10 no metastasis (M0) and 30 distant metastasis (M1) were assessed by sEV-miRNA-sequencing. sEV miRNAs with diverse expression profiles across different metastatic patterns were combined into sigantures to characterize and predict gastric cancer metastasis. Small extracellular miRNAs from 40 gastric cancer plasma samples were sequenced with Illumina NovaSeq platform.