File S1 - Sox17 Regulates Liver Lipid Metabolism and Adaptation to Fasting

Figure S1) (A) Quantification of Vnn3 transcripts by qRT-PCR in liver and spleen of WT and SHIVA mice. (B) Pantetheinase activity (pAMC substrate) in serum from control mice reconstituted with bone marrow from WT or SHIVA mice. Figure S2) Mapping of the Sox17 mutation in (B6xC3H/HeN)F1 backcrosses as described in supporting information. Figure S3) Sox17 expression in liver and cells. (A) Immunohistochemistry analysis of Sox17 expression (FITC) on frozen liver sections (DAPI in white). (B) Immunostaining of Sox17 (red) in COS7 cells (DAPI in blue) following transfection with control or SHIVA Sox17 plasmids (x63). Figure S4) (A) qRT-PCR analysis on two independent cohorts of fed versus fasted control and SHIVA mice: Representative additional qRT-PCR experiments were performed on other transcripts to confirm the microarray analysis and extend the results shown in Figure 2B. Mice were fasted 24 h. Results are organized in broad categories based on their involvement in global functions (Abcd2 and 3 are peroxisomal proteins, some results are not shown to simplify the figure). (B) qRT-PCR on liver samples from 6 days fibrate-treated WT or SHIVA mice. Figure S5) A peroxisomal signature reduced in fasted SHIVA mice. Figure S6) Analysis of liver extracts from fed or fasted WT and SHIVA mice. (A) Quantification of the PMP70 protein by western blot on liver extracts (B) Quantification of catalase and Acox1 activities; (C) Quantification of acot activities using various acyl-CoA FA. Table S1) Oligonucleotides used for qRT-PCR in this study. Table S2) Leading edge of gene list from the GSEA comparing fasted SHIVA versus PPARα-deficient mice. Table S3) Leading edge of gene list from the GSEA comparing fasted SHIVA versus KEGG peroxisome geneset.
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