Genome-wide characterization of TAZ binding sites in mammary epithelial cells in temporary and special manner (RNA-seq)

TAZ, one of the key components of Hippo signaling pathway, is a transcription coactivator and plays an important role in cell proliferation, anti-apoptosis, therapeutic resistance, cancer stemness and tumorigenesis. TAZ mediated transcription program through transcription factors, such as TEAD family members. Genome-wide TAZ occupancy is poorly defined. Here, using a tet-inducible TAZ activation in mammary epithelial MCF10A cells, we characterized genome-wide binding sites in different TAZ activation time points. We found vast majority of TAZ binds to distal enhancer and intergenic regions of genes in the early TAZ activation time point and shift to promoter regions in the later TAZ activation time point. We identified three distinctive TAZ binding patterns to its targets. Furthermore, we found TAZ activation results in chromatin architecture alterations. Our studies provide insight of TAZ regulated transcription program in mammary epithelial cells and indicate potential therapeutic targets for breast cancer. Inducible TAZ-4SA transduced MCF10A cells were treated without Dox or with 0.05µg/ml Dox for 24 h, 48 h and 72 h. Total RNA was extracted using Trizol Reagent (Life Technologies) according to the manufacturer’s protocol. RNAs were further processed for next generation sequencing at Roswell genomic core facility.