Phenotypic and genotypic analysis of 75 Bradyrhizobium strains.

1Strain codes include year of isolation (05 = 2005), host species (LoA = Lotus angustissimus, LoM = L. micranthus, LoH = L. heermannii, LoS = L. strigosus), plant number, and nodule or root-surface number (the latter with R followed by root and isolate number).2Results of greenhouse nodulation assays in which each Bradyrhizobium isolate was tested on 5–8 inoculated seedlings to examine nodulation capability.3Results of PCR amplification assays in which we attempted to amplify each of three symbiosis loci (nifD, nifH, nodDA). Unsuccessful reactions were repeated thrice to confirm lack of amplification.