APLNR marks a multipotent human cardiac progenitor derived with induced pluripotent stem cells

We identified APLNR as a surface marker for in vitro cardiac progenitors derived from human induced pluripotent stem cells (hiPSC). To gain further insight on the differentiation trajectory and its relevance with in vivo cardiac development, we performed polyA mRNA-sequencing on APLNR+ in vitro cardiac progenitors derived from 3 hiPSC lines at 0, 24, 48 and 72 hours post-immunomagnetic isolation. Our study revealed APLNR+ in vitro cardiac progenitors differentiate via a transient progenitor stage before further differentiation into cardiomyocytes and cardiac mesenchyme. Poly-A mRNA from APLNR+ in vitro cardiac progenitors at 0, 24, 48 and 72 hours post-isolation were taken for sequencing.