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Transcription profiling of pancreases of cystic fibrosis mouse with a genotype of gastrin+/-, CFTR-/- to study the gene expression patterns in cystic fibrosis

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Total RNA was prepared using TRIzol reagent from the pancreata of eight week old male mice. The genotypes were Control: gastrin+/-, CFTR+/+; and CF: gastrin+/-, CFTR-/-. All mice were on 95% black6, 5% 129Sv background. Mice were fed Peptamen from age 10 days to prevent intestinal obstruction.

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