Development of a SNP genotyping array for oat and detection of loci related to seedlings low nitrogen tolerance

We developed a target capture sequencing SNP genotyping platform (oat 50K SNP array), and used 440 samples to evaluate the capture quality of this chip. In addition, an association panel consisting of 186 oat accessions was used for a GWAS of genetic loci associated with root and seedling biomass traits following 2 and 0.2 mM NH4NO3 treatments. We have specified that a total of 69 SNPs.The following are supplementary data.IncludingTable S1. Plant Materials and experiments.Table S2. Information for 59000 SNP and probes on the Oat 50K SNP array.Table S3. Chromosome distribution of genome-specific SNP markers.Table S4. Genotyping results for 440 oat accessions.Table S5.The specific detection rate of the 440 oat resources.Table S6. Phenotypic data under HN conditions.Table S7. Phenotypic data under LN conditions.Table S8. Phenotypic description and generalized heritability of each trait of 186 oat accessions.Table S9. Principal component analysis of the low nitrogen tolerance coefficient.Table S10. D value and ranking of comprehensive evaluation of 186 oat materials.Table S11. SNP sites significantly associated with shoot dry weight.Table S12. Candidate genes and functional annotation of low nitrogen tolerance in oat.