Overview of N-terminal acetylation of yeast and human proteins.^*

Quantitative COFRADIC-based analysis of N-terminal acetylation in yeast (S. cerevisiae) and HeLa proteomes determined the acetylation status of 648 and 1345 unique N-termini in the two species, respectively. Overall, 67.9% and 85.2% of the yeast and HeLa proteomes, respectively, are N-terminally acetylated (fully or partially). The analysed proteins are categorized based on their N-terminal sequences (substrate classes). *Only N-termini of which the degree of N-Ac could be univocally calculated/determined in the control yeast (648) and control human (1345) setups were used for the overall calculation of N-Ac.