UNITE
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UNITE is a database and sequence management environment centered on the eukaryotic nuclear ribosomal ITS region. All eukaryotic ITS sequences from the International Nucleotide Sequence Database Collaboration are clustered to approximately the species level (distance between species in steps of 0.5%), and all such species hypotheses are given a DOI to facilitate unambiguous scientific communication and data assembly. The user can interact with these species hypotheses (e.g., SH1566366.08FU) in various ways, and third-party sequence and metadata annotation are supported. Registration is needed to access the more powerful features of the accompanying PlutoF workbench. A range of search and query options are available, and precompiled reference datasets for local sequence similarity searches and HTS pipelines are available for download. Owing to the mycological roots of UNITE, two versions of the release files are available: all eukaryotes (including fungi), and fungi only. UNITE is a community effort that relies on the scientific expertise of its users – please consider contributing by, e.g., annotating sequences of your taxa of expertise.
UNITE是一款以真核生物细胞核核糖体内转录间隔区(Internal Transcribed Spacer,简称ITS)为核心的数据库与序列管理环境。所有来自国际核苷酸序列数据库协作体(International Nucleotide Sequence Database Collaboration, INSDC)的真核生物ITS序列,均以0.5%的物种间差异为聚类步长,被聚类至近似物种水平;所有此类物种假说均被赋予数字对象唯一标识符(Digital Object Identifier,简称DOI),以促进清晰明确的科学传播与数据整合。用户可通过多种方式与这些物种假说(例如SH1566366.08FU)进行交互,同时支持第三方序列及元数据注释。如需使用配套的PlutoF工作台的高级功能,需完成注册。平台提供多样化的搜索与查询选项,同时可下载用于本地序列相似性搜索及高通量测序(High-Throughput Sequencing,简称HTS)流程的预编译参考数据集。鉴于UNITE具有真菌学的起源背景,其发布文件提供两个版本:涵盖所有真核生物(含真菌)的版本,以及仅包含真菌的版本。UNITE是一项依托用户科学专业知识的社区共建项目,恳请广大用户通过为自身研究类群的序列提供注释等方式参与项目建设。




