RNA-seq of adult human spinal cord neural stem/progenitor cells: impact of aging and clinical exposures on proliferation dynamics

Bulk RNA sequencing was performed on neural stem/progenitor cell (NSPC) cultures derived from six adult human spinal cord donors (thoracic, lumbar, and conus regions). Samples were sequenced using the Illumina NovaSeq 6000 platform with paired-end 150 bp reads. Transcript abundances were quantified with Salmon (GENCODE v35 reference). This dataset supports the study “Aging and Clinical Exposures Shape Proliferation Dynamics of Adult Human Spinal Cord Neural Stem/Progenitor Cells,” providing a resource for examining donor- and region-specific transcriptional programs influencing NSPC proliferation. Adult human spinal cord tissue was obtained from organ donors under institutional ethics approval. Neural stem/progenitor cell (NSPC) cultures were established from thoracic, lumbar, and conus regions (six donors, male and female). Total RNA was extracted and libraries were prepared using the Illumina TruSeq Stranded mRNA kit. Libraries were sequenced on an Illumina NovaSeq 6000 (paired-end, 150 bp). Transcript abundances were quantified using Salmon (v1.x) against the GENCODE v35 transcriptome. Processed quantifications and raw FASTQ files are provided.