ATF-4 regulates C. elegans defense against pathogen infection

To gain molecular insights into how ATF-4 regulates C. elegans defense against bacterial infection, we employed RNA-seq to profile gene expression in atf-4(ok576) and WT animals with or without S. enterica infection. Overall design: Four biological replicates of RNA samples from each of four groups of worms (atf-4(ok576) and WT animals with or without exposure to S. enterica for 24 hours) were collected and subjected to RNA-seq analysis.