RNA replication by Q beta replicase: a working model.
收藏PubMed Central1996-10-15 更新2026-05-02 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC38096/
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资源简介：
Two classes of RNA ligands that bound to separate, high affinity nucleic acid binding sites on Q beta replicase were previously identified. RNA ligands to the two sites, referred to as site I and site II, were used to investigate the molecular mechanism of RNA replication employed by the four-subunit replicase. Replication inhibition by site I- and site II-specific ligands defined two subsets of replicatable RNAs. When provided with appropriate 3' ends, ligands to either site served as replication templates. UV crosslinking experiments revealed that site I is associated with the S1 subunit, site II with elongation factor Tu, and polymerization with the viral subunit of the holoenzyme. These results provide the framework for a three site model describing template recognition and product strand initiation by Q beta replicase. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1996-10-15
