Next Generation Sequencing Facilitates Quantitative Analysis of control and MILIP Knockdown by siRNA in A549 human lung cancer cell line. Next Generation Sequencing Facilitates Quantitative Analysis of control and MILIP Knockdown by siRNA in A549 human lung cancer cell line

Enriched p53 signalling after MILIP knockdown was validated by RNA-seq; A c-myc reponsive lncRNA MILIP is commonly upregulated in diverse cancer types. RNA-seq analysis of A549 cells following MILIP knockdown revealed that p53 signalling was the mostly enriched gene pathway, suggesting c-Myc may inactivate p53 through MILIP in cancer. Overall design: For RNA-seq analysis of MILIP siRNA versus control siRNA, the A549 cells were transfected with siRNAs for 48 hours before harvesting.