Modena test datasets: human 18S rRNA, curlcakes, oligos, oligos mixtures

The human 18S rRNA, curlcakes, oligos and oligos mixtures test datasets accompanying the article "Nanopore based computational method for detecting a wide range of epigenetic/epitranscriptomic modifications".

Human 18S rRNA dataset contains a control sample and a native sample. Each sample comprises six subsamples with coverage depths ranging from 10 to 500.

Curlcake-M1 dataset contains a single sample. This sample comprises eight subsamples with coverage depths ranging from 10 to 1000. For each given coverage depth, a subsample contains four modified curlcakes, where each adenine (A) was replaced with m6A. Curlcake-UNM1 dataset contains a single sample. This sample comprises eight subsamples with coverage depths ranging from 10 to 1000. For each given coverage depth, a subsample contains four unmodified curlcakes. Curlcake-M2 dataset contains a single subsample with a coverage depth of 2000. This subsample contains four modified curlcakes, where each adenine (A) was replaced with m6A. Curlcake-UNM2 dataset contains a single subsample with a coverage depth of 2000. This subsample contains four unmodified curlcakes.

Oligo-1, Oligo-2 and Oligo-3 contain three modified samples of the three Oligos variants, while Oligo-C contains three unmodified samples. Each sample comprises eight subsamples with coverage depths ranging from 10 to 2000.

Oligo-mixtures dataset contains 10 samples. Each sample comprises subsamples with coverage depths ranging from 10 to 2000. Additionally, for each sample and coverage depth, there are three different mixtures. Each of these three mixtures has a ratio of modified to unmodified reads of either 25:75, 50:50, or 75:25.

If you have any questions about the content of this dataset, feel free to contact Siniša Biđin at [e-mail].