Table1.XLSX

HIGHLIGHTSMetabolites and transcripts related to plant physiology in salt stress conditions, especially to the recovery process were disclosed in peanut.Peanut (Arachis hypogaea L.) is considered as a moderately salt-sensitive species and thus soil salinity can be a limiting factor for peanut cultivation. To gain insights into peanut plant physiology in response to salt stress and alleviation, we comprehensively characterized leaf relative electrolyte leakage (REC), photosynthesis, leaf transpiration, and metabolism of plants under salt stress and plants that were subjected to salt stress followed by salt alleviation period. As expected, we found that REC levels were higher when plants were subjected to salt stress compared with the untreated plants. However, in contrast to expectations, REC was even higher compared with salt treated plants when plants were transferred from salt stress to standard conditions. To decipher REC variation in response to salt stress, especial during the recovery, metabolite, and transcript variations were analyzed by GC/MS and RNA-seq method, respectively. Ninety two metabolites, among total 391 metabolites identified, varied in response to salt and 42 metabolites responded to recovery specially. Transcriptomics data showed 1,742 in shoots and 3,281 in roots transcript varied in response to salt stress and 372 in shoots and 1,386 transcripts in roots responded specifically to recovery, but not salt stress. Finally, 95 transcripts and 1 metabolite are indicated as candidates involved in REC, photosynthesis, transpiration, and Na+ accumulation variation were revealed by using the principal component analysis (PCA) and correlation analysis. This study provides valuable information on peanut response to salt stress and recovery and may inspire further study to improve salt tolerance in peanut germplasm innovation.

本数据集揭示了在盐胁迫条件下，与植物生理相关的代谢物和转录本信息，尤其是花生（Arachis hypogaea L.）的恢复过程。花生作为一种对盐分中度敏感的物种，土壤盐分常常成为花生栽培的限制性因素。为了深入探究花生植物对盐胁迫及缓解过程的生理反应，我们全面分析了盐胁迫下以及盐胁迫后缓解期的叶片相对电解质渗漏率（REC）、光合作用、叶片蒸腾和植物代谢。预期之中，我们观察到盐胁迫处理下的植物REC水平相较于未处理植物有所升高。然而，出人意料的是，当植物从盐胁迫环境转移到标准条件时，REC水平甚至高于盐处理植物。为了解析盐胁迫特别是恢复过程中的REC变化，我们分别通过GC/MS和RNA-seq技术分析了代谢物和转录本的变化。在总共识别的391种代谢物中，92种在盐胁迫下发生变化，其中42种在恢复过程中特别响应。转录组学数据表明，在盐胁迫下，茎部有1,742个转录本、根部有3,281个转录本发生变化，而在茎部有372个转录本、根部有1,386个转录本专门响应恢复而非盐胁迫。最终，通过主成分分析（PCA）和相关性分析，揭示了95个转录本和1个代谢物作为REC、光合作用、蒸腾作用和Na+积累变化的候选基因。本研究为花生对盐胁迫和恢复的反应提供了宝贵信息，并可能激发进一步研究以提升花生种质资源对盐分的耐受性。