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Data from various stages of the analysis pipeline on coral collected sampled in the Caribbean during 2013 (Contagious coral diseases project)

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DataONE2019-06-11 更新2024-06-08 收录
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<p>This dataset contains analysis pipeline files.&nbsp;There are 3 types of files:</p> <p class=\"rteindent1\">1. One set has the extension “.fa”. These are the fasta files in various stages through our analysis pipeline.&nbsp;&nbsp;</p> <p class=\"rteindent1\">2. Additionally, several chimera checks are performed.&nbsp;Chimera sequences are placed in chimera files e.g. &nbsp;\"fasta.chimeras.fa.\"</p> <p class=\"rteindent1\">3. One file contains a cluster for error correction and output consensus \"cons.fa\"</p> <p>Coral disease transmission experiments were completed for dark-spot syndrome on Sidereastrea siderea&nbsp;and yellow-band disease on Orbicella faveolata, as described in Randall et al. 2016.&nbsp;Following experimentation, microbial communities were extracted from tissue samples to determine whether any&nbsp;potential pathogen may have transmitted from healthy to exposed corals. Microbial communities on healthy corals&nbsp;were compared with diseased corals to identify any potential pathogens.</p> <p>Experimental diseased and healthy corals were sampled and their microbial communities were analyzed using 454 Illumina pyrosequencing&nbsp;of the amplified 16S rRNA gene on the V1-V3&nbsp;hypervariable region.</p>
创建时间:
2021-12-05
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