Table2_Identification of Extracellular Matrix Signatures as Novel Potential Prognostic Biomarkers in Lung Adenocarcinoma.XLSX

The extracellular matrix (ECM) is vital to normal cellular function and has emerged as a key factor in cancer initiation and metastasis. However, the prognostic and oncological values of ECM organization-related genes have not been comprehensively explored in lung adenocarcinoma (LUAD) patients. In this study, we included LUAD samples from The Cancer Genome Atlas (TCGA, training set) and other three validation sets (GSE87340, GSE140343 and GSE115002), then we constructed a three-gene prognostic signature based on ECM organization-related genes. The prognostic signature involving COL4A6, FGA and FSCN1 was powerful and robust in both the training and validation datasets. We further constructed a composite prognostic nomogram to facilitate clinical practice by integrating an ECM organization-related signature with clinical characteristics, including age and TNM stage. Patients with higher risk scores were characterized by proliferation, metastasis and immune hallmarks. It is worth noting that high-risk group showed higher fibroblast infiltration in tumor tissue. Accordingly, factors (IGFBP5, CLCF1 and IL6) reported to be secreted by cancer-associated fibroblasts (CAFs) showed higher expression level in the high-risk group. Our findings highlight the prognostic value of the ECM organization signature in LUAD and provide insights into the specific clinical and molecular features underlying the ECM organization-related signature, which may be important for patient treatment.

细胞外基质（ECM）对于细胞正常功能至关重要，并已成为癌症发生和转移的关键因素。然而，在肺腺癌（LUAD）患者中，与ECM组织相关的基因在预后和肿瘤学价值方面尚未得到全面探索。在本研究中，我们纳入了来自癌症基因组图谱（TCGA，训练集）以及其他三个验证集（GSE87340、GSE140343和GSE115002）的LUAD样本，然后基于与ECM组织相关的基因构建了三个基因的预后特征。涉及COL4A6、FGA和FSCN1的预后特征在训练集和验证集中均表现出强大的稳定性和可靠性。我们进一步通过整合ECM组织相关特征与临床特征，包括年龄和TNM分期，构建了综合预后列线图，以促进临床实践。高风险评分的患者表现出增殖、转移和免疫标志。值得注意的是，高风险组在肿瘤组织中表现出更高的成纤维细胞浸润。因此，据报告由癌症相关成纤维细胞（CAFs）分泌的因子（IGFBP5、CLCF1和IL6）在高风险组中表现出更高的表达水平。我们的研究突出了LUAD中ECM组织特征的临床预后价值，并为揭示ECM组织相关特征背后的具体临床和分子特征提供了见解，这些特征对于患者治疗可能具有重要意义。