Transcriptional profiling of migratory type 2 dendritic cells from draining lymph nodes of WT and STAT6-KO mice.

The signals driving the adaptation of type-2 dendritic cells (DC2s) to diverse peripheral environments remain mostly undefined. We found that differentiation of CD11blow migratory DC2s, a DC2 population unique to the dermis, requires STAT6 and KLF4-dependent IL-13 signaling whereas DC2s in lung and small intestine are STAT6-independent. Overall design: The study contains bulk RNA-seq data and single cell RNA-seq of migratory DC2 sorted from draining lymph nodes of WT or STAT6-KO mice. The bulk RNA-seq data contains 36 samples of migratory XCR1- SIRPa+ DC2 sorted from ear, mesenteric or small intestine draining lymph nodes of uninfected WT or STAT6-KO mice. scRNAseq dataset analysed the transcriptome of 2700 migratory SIRPa+ DC2 from ear draining lymph nodes of WT or STAT6-KO. Supplementary scripts and metadata can be found at the following DOIs: * https://doi.org/10.5281/zenodo.5540103 [Single Cell data browser] * https://doi.org/10.5281/zenodo.5542679 [Data processing scripts for figures]