Transcriptome analysis to characterize DNA methylation changes associated with colorectal carcinogenesis. Homo sapiens

Gene expression analysis of a CRC cell line showing wide-spread DNA hypermethylation (SW48) before and after 5-Aza-Cytidine/Trichostatin A induced DNA demethylation to identify targets of Cytosine methylation-dependent gene silencing. Overall design: Gene expression of SW48 colorectal cancer cell line before and after DNA demethylating 5'-Azacytidine and/or Trichostatin A treatment was analyzed on two Illumina HumanWG-6_V3 microarrays and each 3 replicates were distributed over the two chips.