Effects of calcium binding on the conformation of all EF-hand domains, whose structures are known in the apo-form and with bound Ca2+ ions, and target protein ligands (where they exist).

The RMSD values between the apo-form and the protein with bound Ca2+ ions and the target ligand, are calculated using the back-bone atoms of the amino acids of the clusters, and separately, using all heavy atoms of the same amino acids. The RMSD data are shown for the superposition of clusters I and II separately (groups A–C), and for superposition of the two clusters, cluster I/cluster II, simultaneously (groups D and E).*While groups A–C coincide between Tables 3 and 4, the RMSD calculations (this table) between clusters I and II were made only for the protein structures where the conformation of cluster II does not change upon calcium binding, and thus, proteins from group C were not included in D and E. This was done in order to observe only the inter-cluster conformational change.Effects of calcium binding on the conformation of all EF-hand domains, whose structures are known in the apo-form and with bound Ca2+ ions, and target protein ligands (where they exist).