CoBATCH for high-throughput single-cell epigenomic profiling [EC_CoBATCH]

we develop a robut low input cell and high throughput single cell ChIP method used for both native and fixed in vitro cultured cells and intact tissue we fused Protein A to the N-terminal of Tn5 to generated Protein A-Tn5 fusion enzyme, the ProteinA-Tn5-complex is enriched through specific antibodies to genomic regions and Tn5 generates indexed chromatin fragments ready for the library preparation and sequencing