In situ foliar augmentation of multiple species for optical phenotyping and bioengineering using soft robotics

Precision agriculture aims to increase crop yield while reducing the use of harmful chemicals (e.g., pesticides, excess fertilizer) by employing minimal, tailored interventions. These strategies, however, are limited by (i) sensor quality, which typically relies on visual plant expressions, and (ii) the manual, destructive nature of many non-visual measurement methods, such as the Scholander pressure bomb. By automating more intimate interactions with foliage, in vivo, it would be possible to inject chemical and biological probes that reveal more phenotypes, such as water stress in response to varying environmental conditions, and visible gene expression to measure the success of gene engineering applications. To address this, we developed a soft robotic leaf gripper and stamping-injection method to improve foliar delivery of nanoscale synthetic and biological probes. This allows for non-destructive, in situ, multi-species applications. We used two probes: (i) Agrobacterium tumefaciens ..., , # Data from: In situ foliar augmentation of multiple species for optical phenotyping and bioengineering using soft robotics

Dataset DOI: [10.5061/dryad.9kd51c5vm](10.5061/dryad.9kd51c5vm)

## Description of the data and file structure

Includes:

* PICTURES/MOVIES:
1. Actuator Blocking Force Pictures
2. Actuator Elongation Pictures
3. Actuator Profile Movie
4. Agroinfiltration Pictures
5. Confocal Microscopy Pictures
6. Cotton Injection-Injury Pictures
7. Sunflower Injection-Injury Pictures

- DATASET & MATLAB Scripts:
1. Actuator Optimization Dataset
2. Injection area and porometer Dataset
3. Spectrometer Dataset
4. Spectrophotometer Dataset

### Files and variables

Note: All the folders have respective README file which describes the respective files.

#### File: Actuator_Optimization_Dataset.zip

**Description:** ANSYS simulation results for 800 cases, along with a MATLAB script for generating the surface optimization plot.

#### File: Actuator_Blocking_Force_...,