Comparison of NK cell developmental stages in bone marrow of Tbx21 WT versus KO mice

Bone marrow cells were isolated from wild type and Tbx21 knockout mice. iNK (Lin- NK1.1+ CD11b- CD27+) and mNK1 (Lin- NK1.1+ CD11b+ CD27+) were sorted from WT and Tbx21 KO mice. mNK2 (Lin- NK1.1+ CD11b+ CD27-) were sorted from WT mice only, since these cells are not present in Tbx21 KO mice. Cells from the bone marrow of three WT and three Tbx21 KO mice were sorted. Each of the WT mice yielded iNK, mNK1 and mNK2. Each of the Tbx21 KO mice yielded iNK and mNK1. Total of 15 samples.