Variation of the Intestinal Microbiome during the life course of the Intra-Uterine Growth Restricted Rat Offspring Exposed to a High Fat and Fructose Diet

We reported the variation in the gut microbiome diversity under the influence of Intrauterine Growth restriction or Exposure to High Fat High fructose diet followed by Intrauterine growth restriction. Overall design: We compared four experimental rat groups: 1) Ad lib fed regular chow diet pre- and postnatally (CON), 2-3) IUGR induced by maternal caloric restriction prenatally followed postnatally (PN) by either 2) the control diet (IUGR/RC) or 3) High-Fat-high-fructose (IUGR/HFhf) diet, and lastly 4) HFhf ad lib pre- and postnatally (HFhf). Fecal samples were collected from dams and male and female rat offspring at postnatal day 2, 21, adult at day 180d, and subjected to microbiome analysis by 16S-based rRNA sequencing.