Microbial community structure between water and sediment in artificial reefs detected by primer V4

Marine microorganisms are indispensable regulators of global biogeochemical cycles, while there is little well-know about the microbial community of bottom water and sediment in the artificial reefs which are deploy for enhancement of fishery resources. High-throughput sequencing has facilitated great advances in profiling diversity of microbes, however, the results may vary significantly owing to the choice of primer. This study compared the influence of 16S rRNA universal primers (V4 and V3-V4) on microbial community composition and diversity.