Nectin-4 PET for predicting enfortumab vedotin dose-response in urothelial carcinoma

Optimizing dosing strategies is critical to balance effectiveness and toxicity, especially for drugs with narrow therapeutic windows such as antibody-drug conjugates (ADCs). This study evaluates whether positron emission tomography (PET) imaging targeting Nectin-4 can non-invasively quantify the real-time interaction of the ADC enfortumab vedotin (EV) with tumors in urothelial carcinoma (UC). Using the imaging agent [68Ga]AJ647, dynamic changes in interaction of EV with Necin-4 were measured across preclinical models and correlated with therapeutic responses. PET imaging identified dose-dependent variations in Nectin-4 engagement, with suboptimal EV doses resulting in incomplete Nectin-4 engagement and increased tumor growth. Crucially, PET measured target engagement predicted therapeutic outcomes more reliably than either drug dose or baseline target expression. By defining effective target engagement levels needed for optimal therapeutic outcomes, PET imaging provides a clear benchmar..., , , # Nectin-4 PET for predicting enfortumab vedotin dose-response in urothelial carcinoma

Dataset DOI: [10.5061/dryad.612jm64jr](https://doi.org/10.5061/dryad.612jm64jr)

## **Overview**

This README outlines the contents of files uploaded to Dryad for the study titled \"Nectin-4 PET for predicting enfortumab vedotin dose-response in urothelial carcinoma.\" All required files are contained within the zip file “ROC_Curves_and_Response_prediction.zip\".

### **1. Data File: 20240502_responder_prediction.xlsx**

* **Description:** This file contains tumor volume (mm3) measurements for preclinical mouse models over time, structured to predict response using the modified RECIST criteria.
* **File Structure:**
* Each column (except the first) represents an individual mouse.
* The first column contains day numbers, and the first row identifies mouse IDs.
* **Variables:**
* **Day (Column 1):** Time points for tumor volume measurements. Unit for tumor volume is mm3.
* **Mouse IDs (Row 1):** I...