Detoxification of aflatoxin B1 by essential oils of medicinal herbs

The essential oils (EO) extracted from the medicinal plants viz., Heliotropium bacciferum, Ocimum dhofarense and Zataria multiflora were diluted with methanol (1:10, v/v) and 250 μl of EO was mixed with AFB1 (50 μg/l) in a microcentrifuge. Following a 24-h incubation at 25°C, AFB1 in the mixture was extracted with 250 μl of chloroform. The chloroform fraction was evaporated at 60°C using a water bath and the residue was quantified by enzyme-linked immunosorbent assay using a commercial kit (RIDASCREEN Aflatoxin B1; R-Biopharm AG, Darmstadt, Germany).

从药用植物如天芥菜（Heliotropium bacciferum）、罗勒（Ocimum dhofarense）和毛莨香（Zataria multiflora）中提取的精油（EO）以甲醇（1:10，体积比）稀释，并将250 μl的精油与AFB1（50 μg/l）在微量离心管中混合。在25°C条件下孵育24小时后，利用250 μl的氯仿从混合物中提取AFB1。使用水浴在60°C下蒸发氯仿部分，并通过酶联免疫吸附测定法（使用商业试剂盒 RIDASCREEN Aflatoxin B1；R-Biopharm AG，达姆施塔特，德国）对残留物进行定量。