aBHQ1, black hole quencher-1; 6FAM, 6-carboxyfluorescein; JOE, 6-carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein. bITS, internal transcribed spacer. Primers and probes used for multiplex quantitativ
The denature, anneal and extension steps were cycled 32 times. *Fluorescence signal was read in the SYBR channel at the end of each cycle during the cycling phase of the assay, and throughout the melt