Localization of recombination proteins and Srs2 reveals anti-recombinase function in vivo

Cells containing Rad52-RFP and YFP-Srs2 and a PCNA mutant that is not able to be sumoylated were imaged through 11 z-sections at 0.3µm intervals. In small-budded (S phase) cells, Srs2 does not form multiple small replication foci. However, Srs2 retains the ability to colocalize with Rad52 in recombination foci. To view DIC only, turn off Channels 1 and 2. Turn on Channel 0. For grayscale, uncheck the color" box (above "zoom")."