Optimisation of HTT-HAP40 purification using heparin affinity chromatography - 2019/04/02

<strong>Project: </strong>Biophysical investigation of purified HTT protein samples <strong>Experiment: </strong>Optimisation of HTT-HAP40 purification using heparin affinity chromatography <strong>Date completed:­ </strong>2019/04/02 <strong>Rationale: </strong>Previous attempts to generate a much purer and homogenous HTT-HAP40 sample showed that the complex can bind heparin resin. In addition, I will incorporate helpful suggestions from scientists at the CHDI Palm Springs meeting full-length HTT research breakout group i.e. ATP wash to remove HSP proteins. The experiment will include 3 affinity chromatography steps with FLAG, heparin and NiNTA resin and finally a gel filtration step.